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Tumours derived from HTLV‐I tax transgenic mice are characterized by enhanced levels of apoptosis and oncogene expression
Author(s) -
Hall Anthony P.,
Irvine Jane,
Blyth Karen,
Cameron Ewan R.,
Onions David E.,
Campbell Moyra E. M.
Publication year - 1998
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(1998100)186:2<209::aid-path162>3.0.co;2-i
Subject(s) - apoptosis , oncogene , genetically modified mouse , transgene , expression (computer science) , cancer research , biology , genetics , gene , cell cycle , computer science , programming language
Abstract In order to investigate the role that the human T‐lymphotropic virus type I (HTLV‐I) tax oncogene plays in apoptosis and transformation in vivo , four lines of HTLV‐I tax transgenic mice were generated under the regulatory control of the CD3‐ϵ promoter–enhancer sequence. These mice develop a variety of phenotypes including mesenchymal tumours, which develop at wound sites, and salivary and mammary adenomas. In situ DNA fragment labelling and immunocytochemical analysis of these tumours reveals that they display enhanced levels of apoptosis, which is associated with elevated levels of Myc, Fos, Jun, and p53 protein expression. Furthermore, double immunofluorescent staining shows that Tax expression and apoptosis co‐localize, indicating that Tax expression is closely associated with apoptosis in vivo . Copyright © 1998 John Wiley & Sons, Ltd.