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Immunohistochemical detection of ribosomal transcription factor UBF: diagnostic value in malignant specimens
Author(s) -
TorresMontaner Antonio,
Bolivar Jorge,
Ortiz Manuela,
Valdivia Manuel M.
Publication year - 1998
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199801)184:1<77::aid-path958>3.0.co;2-x
Subject(s) - nucleolus organizer region , biology , silver stain , interphase , staining , microbiology and biotechnology , immunohistochemistry , immunofluorescence , nucleolus , ribosomal rna , pathology , nucleolar organizer region , transcription (linguistics) , antibody , gene , immunology , genetics , nucleus , medicine , linguistics , philosophy
The nucleolar organizer regions (NORs) of human chromosomes can be identified in interphase and mitotic cells by localization of some intrinsic components such as the associated enzyme RNA polymerase I. A new sensitive staining method for NORs is described using a specific antibody to the ribosomal transcription factor UBF. By indirect immunofluorescence and enzyme‐labelling methods, NORs stained in benign and malignant cells from a variety of tissues with monospecific anti‐UBF serum showed significant morphological differences which correlated well with histopathological evaluation. The number of NORs per cell in malignant preparations increased significantly. Furthermore, the staining of a NOR protein component such as UBF appears to be as sensitive as the silver‐staining technique (AgNOR) and might be a better alternative for detecting ribosomal activity in malignant tissues. © 1998 John Wiley & Sons, Ltd.