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APC EXPRESSION IN NORMAL HUMAN TISSUES
Author(s) -
MIDGLEY CAROL A.,
WHITE SUSAN,
HOWITT RACHEL,
SAVE VICKI,
DUNLOP MALCOLM G.,
HALL PETER A.,
LANE DAVID P.,
WYLLIE ANDREW H.,
BUBB VIVIEN J.
Publication year - 1997
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199704)181:4<426::aid-path768>3.0.co;2-t
Subject(s) - adherens junction , immunohistochemistry , biology , microbiology and biotechnology , cytoplasm , epithelium , staining , epitope , antibody , cell , immunology , genetics , cadherin
The tumour suppressor gene APC codes for a 2843‐amino acid protein whose precise functions are still poorly understood. This paper describes the development of two new antisera to APC (to amino‐ and carboxy‐terminal epitopes) which permit localization of the protein by immunohistochemistry in archival paraffin sections. The protein is expressed in a wide variety of normal epithelial tissues. Its distribution frequently coincides with the location of post‐replicative cells within tissues. Staining patterns demonstrate that the APC protein, although often diffusely cytoplasmic in distribution, may also accumulate in the apical and immediately subapical regions, or along the lateral margins of certain cells. These results indicate that APC is significant in many tissues in addition to the colorectal epithelium. They are compatible with a function related to signalling at the adherens junction and possibly with other more complex roles in cells committed to terminal differentiation. © 1997 by John Wiley & Sons, Ltd.