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APOPTOSIS OF HUMAN MONOCYTES/MACROPHAGES IN MYCOBACTERIUM TUBERCULOSIS INFECTION
Author(s) -
PLACIDO ROBERTA,
MANCINO GIORGIO,
AMENDOLA ALESSANDRA,
MARIANI FRANCESCA,
VENDETTI SILVIA,
PIACENTINI MAURO,
SANDUZZI ALESSANDRO,
BOCCHINO MARIA LUISA,
ZEMBALA MAREK,
COLIZZI VITTORIO
Publication year - 1997
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199701)181:1<31::aid-path722>3.0.co;2-g
Subject(s) - apoptosis , mycobacterium tuberculosis , tuberculosis , tunel assay , propidium iodide , macrophage , immunology , alveolar macrophage , bronchoalveolar lavage , microbiology and biotechnology , medicine , biology , virology , programmed cell death , in vitro , pathology , lung , biochemistry
Tuberculosis (TB) is still a major health problem, both as a single disease entity and as a cofactor in AIDS. The interaction between macrophage and Mycobacterium tuberculosis (MTB) is a critical step in the establishment of an early chronic infection. This study analyses the capacity of MTB to induce apoptosis in cells obtained by broncho‐alveolar lavage (BAL) from patients with reactive pulmonary tuberculosis and from AIDS patients with disseminated pulmonary tuberculosis. Apoptosis was increased three‐fold in BAL cells obtained from patients with pulmonary tuberculosis and even more markedly in alveolar macrophages of MTB‐infected AIDS patients, compared with controls. Apoptosis was analysed and characterized by propidium iodide (PI) incorporation, terminal deoxy transferase (TDT)‐mediated dUTP‐biotin nick end labelling (TUNEL), and tissue transglutaminase (tTG) expression. The MTB–macrophage interaction was also investigated in vitro by infecting monocyte‐derived macrophages (MDM) with MTB (virulent strain H37Rv). The induction of apoptosis by MTB required viable bacteria, was dose‐dependent, and was restricted to H37Rv. Infection with either Mycobacterium avium complex (MAC) or HIV‐1 and treatment with heat‐killed MTB failed to induce apoptosis. © 1997 by John Wiley & Sons, Ltd.