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CELL ADHESION MOLECULE EXPRESSION WITHIN HUMAN GLOMERULAR AND KIDNEY ORGAN CULTURE
Author(s) -
SAVAGE CAROLINE O. S.,
BROOKS CHRISTOPHER J.,
ADU DWOMOA,
RICHARDS GERALDINE,
HOWIE ALEXANDER J.
Publication year - 1997
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199701)181:1<111::aid-path698>3.0.co;2-9
Subject(s) - cell adhesion molecule , e selectin , biology , intercellular adhesion molecule 1 , cytokine , renal glomerulus , tumor necrosis factor alpha , kidney , microbiology and biotechnology , cell adhesion , glomerulonephritis , immunology , endocrinology , cell , biochemistry
A glomerular and kidney organ culture method was developed to study cytokine inducibility of adhesion molecules and MHC antigen expression. Glomerular cells showed constitutive expression of intercellular cell adhesion molecule‐1 (ICAM‐1) and MHC class I and II antigens, but not vascular cell adhesion molecule‐1 (VCAM‐1), E‐selectin, or P‐selectin. Expression of E‐selectin on glomerular endothelial cells (ECs) was induced by interleukin‐1 (IL‐1), tumour necrosis factor (TNF), interferon‐γ (IFN‐γ) and granulocyte/macrophage‐colony stimulating factor (GM‐CSF); this induction was inhibited by transforming growth factor beta (TGFβ) and by IL‐4. P‐selectin expression was never seen within glomeruli. VCAM‐1 was constitutively expressed by Bowman's capsule and proximal tubules and was weakly induced on glomerular ECs by TNF and IL‐4 in combination. Glomerular endothelial ICAM‐1 expression was increased by IL‐1, TNF, IFN‐γ, and GM‐CSF, while TGFβ inhibited induction by TNF and IL‐1. Expression of MHC class I and II antigens by glomerular ECs was constitutive; further upregulation of MHC class II by IFN‐γ was observed. These studies suggest that leukocyte–endothelial cell interactions that occur within the kidney follow broadly similar principles as are proposed to occur elsewhere in the body but, in addition, there are subtle differences that reflect local conditions. © 1997 by John Wiley & Sons, Ltd.