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DIFFERENTIAL GROWTH OF N‐ AND S‐TYPE HUMAN NEUROBLASTOMA CELLS XENOGRAFTED INTO SCID MICE. CORRELATION WITH APOPTOSIS
Author(s) -
PIACENTINI MAURO,
PIREDDA LUCIA,
STARACE DONATELLA,
ANNICCHIARICOPETRUZZELLI MARGHERITA,
MATTEI MAURIZIO,
OLIVERIO SERAFINA,
GRAZIA FARRACE MARIA,
MELINO GERRY
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199612)180:4<415::aid-path684>3.0.co;2-a
Subject(s) - apoptosis , neuroblastoma , cell culture , biology , transfection , cell growth , phenotype , cancer research , cell type , microbiology and biotechnology , programmed cell death , cell , gene , genetics
This study concerns the role of apoptosis in the growth of human neuroblastomas transplanted into immunodeficient SCID mice. Human neuroblastoma cell lines may consist of one or more distinct phenotypes including the neural ‘N‐type’ and flat substrate‐adherent ‘S‐type’. A differential phenotype‐specific proliferation was apparent among S‐ and N‐type cell clones transplanted into SCID mice when compared with the wild‐type SK‐N‐BE(2) cell line. This differential growth capacity of the tumours was correlated with spontaneous apoptosis. Another SK‐N‐BE(2)‐derived cell line (TGA), displaying high levels of apoptosis upon stable transfection with the full length ‘tissue’ transglutaminase (tTG) cDNA, was unable to induce tumour development when xenografted into SCID mice. To support these observations, the expression of apoptosis‐related genes (i.e., bcl‐2, p53, and tTG) in the various neuroblastomas was also investigated.