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c‐Src PROTEIN EXPRESSION IS INCREASED IN HUMAN BREAST CANCER. AN IMMUNOHISTOCHEMICAL AND BIOCHEMICAL ANALYSIS
Author(s) -
VERBEEK BIANCA S.,
VROOM THEA M.,
ADRIAANSENSLOT SABRINA S.,
OTTENHOFFKALFF ASTRID E.,
GEERTZEMA JAN G. N.,
HENNIPMAN ADRIAAN,
RIJKSEN GERT
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199612)180:4<383::aid-path686>3.0.co;2-n
Subject(s) - immunohistochemistry , proto oncogene tyrosine protein kinase src , breast cancer , cancer , biology , pathology , cancer research , medicine , phosphorylation , microbiology and biotechnology
In human breast cancer, c‐Src activity is elevated compared to normal breast tissue. It is not yet known whether this increase in c‐Src activity is accompanied by an increase in c‐Src protein expression. In this study, c‐Src activity and protein expression were determined in a series of human breast cancers and in normal breast tissue, using immune complex kinase assays and immunoblotting. As the heterogeneity of breast cancer is not taken into account in these biochemical experiments, immunohistochemistry was also used to distinguish between normal and malignant cells. In human breast cancers, the c‐Src activity is increased 4‐ to 30‐fold, compared with normal breast tissue. This enhanced activity is accompanied by an increase in c‐Src protein expression, as shown by both immunoblotting and immunohistochemistry. Immunohistochemistry indicates that the majority of c‐Src appears to be concentrated around the nucleus in malignant cells, whereas in normal cells, it is distributed more evenly in the cytoplasm. These data confirm that c‐Src activity is increased in human breast cancer. In addition, this study provides strong immunohistochemical evidence that the c‐Src protein is also overexpressed, enabling a distinction to be made between normal and malignant cells.