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PROGNOSTIC SIGNIFICANCE OF p53 ABNORMALITIES IN COLORECTAL CARCINOMA DETECTED BY PCR–SSCP AND IMMUNOHISTOCHEMICAL ANALYSIS
Author(s) -
LEAHY DERMOT T.,
SALMAN REEM,
MULCAHY HUGH,
SHEAHAN KIERAN,
O'DONOGHUE DIARMUID P.,
PARFREY NOLLAIG A.
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199612)180:4<364::aid-path683>3.0.co;2-0
Subject(s) - immunohistochemistry , single strand conformation polymorphism , exon , colorectal cancer , antigen retrieval , biology , pathology , antigen , p53 protein , tumor suppressor gene , monoclonal antibody , carcinoma , survival analysis , gene , antibody , cancer research , cancer , medicine , carcinogenesis , immunology , genetics
Abnormalities in the p53 tumour suppressor gene and in the expression of its protein are common in colorectal carcinoma. The prognostic significance of these p53 abnormalities was studied in 66 patients with colorectal cancer, followed for more than 10 years. Single‐strand conformation polymorphism (SSCP) analysis was used to detect alterations in exons 5–8 of the p53 gene. Paraffin sections were examined immunohistochemically for p53 overexpression with the monoclonal antibody DO‐7 (Dako) both with and without microwave antigen retrieval. Abnormalities of the p53 gene were found in 41 per cent of cases by SSCP analysis. Outcome was unrelated to SSCP abnormalities ( P =0·19), except for the Dukes' A and B subgroup, where decreased survival was found in cases with abnormal SSCP ( P =0·01). Overexpression of p53 protein was seen by immunohistochemistry in 47 per cent of cases without, and in 52 per cent of cases with microwave antigen retrieval. Immunohistochemical overexpression of p53 protein either with or without microwave antigen retrieval was an independent prognostic indicator of poor survival. These results suggest that for routine purposes, immunohistochemical detection of the p53 protein product may be more useful than SSCP analysis of the encoding p53 gene in identifying those at high risk of colorectal cancer recurrence and death.