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E‐SELECTIN EXPRESSION IN EXPERIMENTAL MODELS OF INFLAMMATION IN MICE
Author(s) -
HENSELEIT UTE,
STEINBRINK KERSTIN,
GOEBELER MATTHIAS,
ROTH JOHANNES,
VESTWEBER DIETMAR,
SORG CLEMENS,
SUNDERKÖTTER CORD
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199611)180:3<317::aid-path670>3.0.co;2-o
Subject(s) - endothelium , e selectin , in vivo , cytokine , inflammation , cell adhesion molecule , l selectin , immunology , tumor necrosis factor alpha , microbiology and biotechnology , selectin , endothelial stem cell , pathology , in vitro , biology , chemistry , medicine , cell adhesion , cell , endocrinology , biochemistry
E‐selectin (CD62E, formerly termed ELAM‐1) is a cytokine‐inducible adhesion molecule which mediates the binding of neutrophils, monocytes, and skin homing T‐cells. The murine homologue of E‐selectin has been cloned. A monoclonal antibody (21KC10) was used here to study immunohistochemically the expression and regulation of murine E‐selectin in vitro and in vivo . As described for the human system, there was no staining of normal endothelium in skin and other tissues. LPS and tumour necrosis factor‐alpha (TNF‐α ), but not interleukin‐4 (IL‐4) or interferon‐gamma (IFN‐ γ), induced a transient expression of E‐selectin, both when injected in vivo and when added to endothelial cell lines in vitro. To analyse temporal expression of E‐selectin under pathophysiological conditions in vivo, we chose two murine models of inflammation: allergic (ACD) and irritant contact dermatitis (ICD). Expression of E‐selectin was found to be induced on vascular endothelium of post‐capillary venules in both ACD and ICD. In ICD, maximal staining of endothelial cells occurred earlier than in ACD. Expression of E‐selectin during ICD and ACD was then compared between strains of mice which differ with regard to the intensity of their inflammatory reaction. BALB/c mice, which in contrast to C57BI/6 mice show a denser infiltrate and prolonged influx of granulocytes and monocytes, revealed a more pronounced and more prolonged expression of E‐selectin than C57BI/6 mice. This held true for both ACD and ICD, and in each case, peak expression of E‐selectin was associated with the highest density of the leukocytic infiltrate. This study thus reveals regulatory mechanisms involved in the expression of murine E‐selectin in vivo and in vitro . It also demonstrates a correlation between endothelial expression of E‐selectin and the genetically determined intensity of the inflammatory response.