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ENHANCED MALIGNANT PROGRESSION OF NASOPHARYNGEAL CARCINOMA CELLS MEDIATED BY THE EXPRESSION OF EPSTEIN–BARR NUCLEAR ANTIGEN 1 IN VIVO
Author(s) -
SHEU LAIFA,
CHEN ANN,
MENG CHINGLIANG,
HO KUOCHIEH,
LEE WEIHWA,
LEU FURJIANG,
CHAO CHUNGFAYE
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199611)180:3<243::aid-path655>3.0.co;2-7
Subject(s) - nasopharyngeal carcinoma , epstein–barr virus , biology , antigen , cancer research , virus , cell culture , carcinoma , in vivo , metastasis , immunology , pathology , cancer , medicine , radiation therapy , genetics , microbiology and biotechnology
Nasopharyngeal carcinoma (NPC) is consistently associated with Epstein–Barr virus (EBV) and mostly classified as poorly differentiated squamous cell carcinoma or undifferentiated carcinoma with early metastasis and a rapidly progressive clinical course. The EBV‐encoded latent proteins, Epstein–Barr nuclear antigen 1 (EBNA 1) and latent membrane proteins (LMPs), may be expressed in NPC, but their biological effects are poorly understood. EBNA 1 may predispose B lymphocytes to lymphomagenesis in transgenic mice, but its biological effects in NPC are still unknown. This study investigated the biological effects of EBNA 1 by expressing it in an EBV‐negative NPC cell line (HONE‐1), which was then inoculated into both nude and severe combined immunodeficiency mice. The EBNA 1 caused HONE‐1 cells to grow in a less differentiated pattern and to progress more rapidly, as well as increasing their tumourigenicity and metastatic capability. These data suggest that EBNA 1 may play a critical role in the progressive evolution of NPC.