Premium
AN ORGAN‐CULTURE METHOD FOR HUMAN COLORECTAL MUCOSA USING SERUM‐FREE MEDIUM
Author(s) -
MOORGHEN M.,
CHAPMAN M.,
APPLETON D. R.
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199609)180:1<102::aid-path613>3.0.co;2-s
Subject(s) - crypt , bromodeoxyuridine , explant culture , organ culture , in vitro , labelling , cell growth , biology , cell culture , intestinal mucosa , epithelium , pathology , microbiology and biotechnology , medicine , endocrinology , biochemistry , genetics
This study describes an explant organ‐culture system in which human colonic mucosa can be maintained for prolonged periods in serum‐free medium. Following an initial phase of epithelial cell loss, there was intense regenerative activity, with the reformation of tubular crypts. Estimation of crypt lengths revealed a marked reduction after 5 and 9 days in culture with corresponding increases in labelling indices for the whole crypt. The shapes of bromodeoxyuridine (BrdU)‐flash‐labelling distribution curves were consistent with a proliferative compartment situated within the lower two‐thirds of the crypt. We conclude that this is a useful in vitro model for the study of the effects of growth factors and growth‐inhibitory agents in respect of cell proliferation in human colonic mucosa.