Premium
THE EFFECT ON CHICK OSTEOCLASTS OF INFECTION WITH PARAMYXOVIRUSES
Author(s) -
SHEPARD SARAH L.,
COOPER ROBERT J.,
McCLURE JOHN
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199608)179:4<448::aid-path636>3.0.co;2-p
Subject(s) - virus , measles virus , paget's disease of bone , canine distemper , osteoclast , bone resorption , resorption , virology , biology , acid phosphatase , tartrate resistant acid phosphatase , antibody , pathology , measles , immunology , medicine , disease , endocrinology , enzyme , biochemistry , vaccination , in vitro
The detection of virus in osteoclasts from Pagetic patients is now well known, but it has yet to be shown convincingly that the presence of virus in Pagetic osteoclasts influences their behaviour. In this study, osteoclasts from embryonic chick tibiae were infected with canine distemper virus or measles virus and compared with mock‐infected controls. Infection was confirmed using virus‐specific fluorescent antibodies. It was found that virus infection did not alter osteoclast morphology or tartrate‐resistant acid phosphatase (TRAP) activity. Both infected and mock‐infected osteoclasts produced resorption pits on bovine bone slices; these could be divided into two distinct size classes with a computer‐based measuring system. Virus infection significantly increased the proportion of the larger size class of resorption pit. These results suggest that virus infection can increase bone resorption by osteoclasts, lending further support to the hypothesis that viruses play a role in Paget's disease of bone.