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MOLECULAR ANALYSIS OF THE NPM – ALK REARRANGEMENT IN HODGKIN'S DISEASE
Author(s) -
XERRI LUC,
PARC PATRICIA,
HASSOUN JACQUES,
BIRNBAUM DANIEL
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199602)178:2<128::aid-path444>3.0.co;2-h
Subject(s) - biology , anaplastic large cell lymphoma , fusion gene , polymerase chain reaction , microbiology and biotechnology , gene rearrangement , histogenesis , reverse transcriptase , gene , cancer research , lymphoma , pathology , genetics , immunohistochemistry , immunology , medicine
The fusion gene NPM – ALK occurs in a subset of anaplastic large cell lymphomas (ALCLs), as a result of a chromosomal translocation, t(2;5) (p23;q35). It has been suggested that Hodgkin's disease (HD) and ALCL share a common histogenesis because of pathological and phenotypical similarities. In order to check this hypothesis, reverse transcriptase‐polymerase chain reaction (RT‐PCR) was performed to detect the hybrid NPM – ALK gene in 30 tumour samples, including 22 lymph node biopsies from HD and eight ALCL specimens. The threshold level of sensitivity was shown to reach at least 1/10 4 by dilution experiments using cell lines as positive and negative controls. The expected 177 bp product indicative of the NPM – ALK rearrangement was identified in Karpas 299 and SUDHL‐1 cell lines and in two out of eight ALCLs. The 22 HD cases were negative, even after two successive tests. Thus, since the ALCL‐specific genetic alteration was absent in our series of HD cases, the present study does not support the hypothesis that HD and ALCL are histogenetically related entities.