Premium
p53 OVEREXPRESSION AND HUMAN PAPILLOMAVIRUS INFECTION IN TRANSITIONAL CELL CARCINOMA OF THE URINARY BLADDER: CORRELATION WITH HISTOLOGICAL PARAMETERS
Author(s) -
TENTI PATRIZIA,
ZAPPATORE RITA,
ROMAGNOLI SOLANGE,
CIVARDI EMILIO,
GIUNTA PAULO,
SCELSI ROBERTO,
STELLA GIORGIO,
CARNEVALI LUCIANO
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199601)178:1<65::aid-path451>3.0.co;2-w
Subject(s) - medicine , hpv infection , immunostaining , immunohistochemistry , transitional cell carcinoma , human papillomavirus , pathology , urinary bladder , urinary system , polymerase chain reaction , monoclonal antibody , antibody , cervical cancer , bladder cancer , biology , cancer , immunology , gene , biochemistry
Seventy‐nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40·5 per cent of the cases; the percentage of positive cases was significantly lower in low‐grade (G1 and G2) TCCs than in high‐grade (G3) tumours (10·6 per cent vs. 84·4 per cent; P <0·0001). The overall rate of HPV infection was 32·9 per cent; 20·3 per cent of the cases were positive for HPV 16, 3·8 per cent for HPV 18, and 8·9 per cent for both. Consensus primers as well as type‐specific primers for HPV types 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low‐grade than in high‐grade tumours (44·7 per cent vs. 15·6 per cent; P =0·0061). p53‐positive cases were more common among papillary, deeply infiltrating tumours, and HPV‐positive cases among papillary, non‐infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low‐grade and in invasive high‐grade tumours is suggested.