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EXPRESSION OF NUCLEOPHOSMIN/B23 IN NORMAL AND NEOPLASTIC COLORECTAL MUCOSA
Author(s) -
NOZAWA YOSHIHIRO,
VAN BELZEN NICO,
VAN DER MADE ANGELIQUE C. J.,
DINJENS WINAND N. M.,
BOSMAN FRED T.
Publication year - 1996
Publication title -
the journal of pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.964
H-Index - 184
eISSN - 1096-9896
pISSN - 0022-3417
DOI - 10.1002/(sici)1096-9896(199601)178:1<48::aid-path432>3.0.co;2-y
Subject(s) - nucleophosmin , pathology , immunohistochemistry , biology , carcinogenesis , cancer research , medicine , cancer , genetics , myeloid leukemia
Nucleophosmin/B23 is a 38 kD molecular phosphoprotein involved in ribosome assembly and transport. In view of the fact that nucleophosmin/B23 appears to be more abundant in tumour cells than in normal cells, the mRNA expression and immunohistochemical localization of nucleophosmin/B23 were investigated in 19 samples of non‐neoplastic mucosa, six adenomas, and 16 adenocarcinomas of the colorectum. Northern blot analysis revealed that nucleophosmin/B23 mRNA is expressed at a higher level in adenomas and carcinomas than in non‐neoplastic mucosa of the colorectum. Immunohistochemical staining of formalin‐fixed, paraffin‐embedded tissue sections after microwave antigen retrieval, using a nucleophosmin/B23‐specific monoclonal antibody, showed almost exclusively diffuse nuclear reactivity of a majority of the epithelial cells in non‐neoplastic mucosa: in adenomas, reactivity was almost exclusively nucleolar and in carcinomas, nuclear as well as nucleolar staining was observed. During mitosis, the immunoreactivity of nucleophosmin/B23 appears in the cytoplasm. The results indicate that the expression of nucleophosmin/B23 is higher in neoplastic than in non‐neoplastic colorectal mucosa. Furthermore, the pattern of nucleophosmin/B23 expression shifts from nuclear to nucleolar early in the adenoma–carcinoma sequence. The exact function of nucleophosmin/B23 in colorectal carcinogenesis remains to be determined.