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Confirmation of the structure of lipid A from Enterobacter agglomerans by electrospray ionization tandem mass spectrometry
Author(s) -
Boué Stephen M.,
Cole Richard B.
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(200003)35:3<361::aid-jms943>3.0.co;2-d
Subject(s) - chemistry , tandem mass spectrometry , electrospray ionization , moiety , mass spectrometry , lipid a , electrospray , chromatography , stereochemistry , bacteria , biology , genetics
Electrospray ionization (ESI) combined with tandem mass spectrometry (MS/MS) was utilized for the structural confirmation of lipid A derived from Enterobacter agglomerans , a Gram‐negative bacterium commonly found in field cotton. Previous ESI‐MS studies conducted in our laboratory found that similarities exist between the fatty acid side‐chains in the lipid A of E. agglomerans and that of Salmonella minnesota . It was noted that heterogeneity at the fatty acyl chain at position 3′ of the diglucosamine backbone of E. agglomerans can take the form of either a myristyloxymyristyl group or, less commonly, a hydroxymyristyloxymyristyl moiety. In this work, tandem mass spectra obtained from heptaacyl and hexaacyl lipid A precursors derived from E. agglomerans and a known standard S. minnesota were compared to assist in structural elucidation. These ESI‐MS/MS experiments confirmed the previously reported structure for lipid A derived from E. agglomerans . Moreover, MS/MS data indicated that the additional hydroxyl group of the 3′‐position hydroxymyristyloxymyristyl moiety is present as the α ‐isomer. Copyright © 2000 John Wiley & Sons, Ltd.