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Antisense peptide interactions studied by electrospray ionization mass spectrometry
Author(s) -
Madhusudanan K. P.,
Katti S. B.,
Haq W.,
Misra P. K.
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(200002)35:2<237::aid-jms934>3.0.co;2-3
Subject(s) - chemistry , peptide , electrospray ionization , sense (electronics) , mass spectrometry , electrospray , protein mass spectrometry , chromatography , biochemistry
Non‐covalent interactions between met‐ and leu‐enkephalins and their antisense peptides were studied by electrospray ionization mass spectrometry. Mixtures of sense and antisense peptides gave both the corresponding homodimers and heterodimers. The relative abundance ratios of the heterodimer to that of the homodimer of the sense peptide and the relative stability constants of the heterodimers were compared with the corresponding values from mixtures of the sense peptides and a control peptide. The results show that there is a preferential interaction between the sense and antisense peptides compared with that between the sense and control peptides. Copyright © 1999 John Wiley & Sons, Ltd.