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Determination of pentazocine in human whole blood and urine by gas chromatography/surface ionization organic mass spectrometry
Author(s) -
Seno Hiroshi,
Kumazawa Takeshi,
Ishii Akira,
Matsushima Hajime,
WatanabeSuzuki Kanako,
Suzuki Osamu
Publication year - 2000
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(200001)35:1<33::aid-jms909>3.0.co;2-t
Subject(s) - chemistry , pentazocine , chromatography , mass spectrometry , urine , gas chromatography , gas chromatography–mass spectrometry , selected ion monitoring , detection limit , whole blood , analytical chemistry (journal) , analgesic , pharmacology , medicine , biochemistry , immunology , biology
Pentazocine has been found to be measurable with much higher sensitivity by gas chromatography (GC)/surface ionization (SI) organic mass spectrometry (OMS) than by the conventional GC/electron ionization (EI) mass spectrometry. The compound was extracted from human whole blood and urine with Sep‐Pak C 18 cartridges before analysis by GC/SIOMS; recoveries were > 96.6% for both samples. The calibration curves were linear in the range 6.25–100 ng ml −1 and the detection limits were 500 pg ml −1 of a sample by selected ion monitoring (SIM) with GC/SIOMS. The intra‐ and inter‐day relative standard deviations for the determination of pentazocine in whole blood and urine were not greater than 9.6%. The sensitivity for pentazocine obtained by SI‐SIM was about 60 times higher than that obtained by EI‐SIM. To validate the present GC/SIOMS method for pentazocine, whole blood and urine samples collected from two volunteers 1–6 h after intramuscular injection of 15 mg of pentazocine were analyzed. The concentrations were 13.5–59.3 ng ml −1 for whole blood and 0.39–4.00 µg ml −1 for urine. Copyright © 2000 John Wiley & Sons, Ltd.