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Measurement of polymorphic trinucleotide repeats in the androgen receptor gene by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Wada Yoshinao,
Mitsumori Kenji,
Terachi Toshiro,
Ogawa Osamu
Publication year - 1999
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199908)34:8<885::aid-jms846>3.0.co;2-u
Subject(s) - chemistry , mass spectrometry , microsatellite , ionization , microbiology and biotechnology , polymerase chain reaction , androgen receptor , chromatography , trinucleotide repeat expansion , matrix assisted laser desorption/ionization , molecular mass , gene , analytical chemistry (journal) , genetics , ion , desorption , biochemistry , biology , allele , enzyme , organic chemistry , prostate cancer , cancer , adsorption
Trinucleotide repeats are polymorphic in normal individuals. CAG repeats in the X‐linked androgen receptor gene were counted by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI/TOF‐MS). A region of approximately two hundred base‐pairs containing the repeats was amplified by polymerase chain reaction, then measured after a simple purification procedure. The single‐charged molecular ion species was detected using 0.1 pmol of DNA sample and the number of repeats was determined from the molecular mass. The results indicated that MALDI/TOF‐MS is a high‐throughput alternative to polyacrylamide gel electrophoresis for precise determination of polymorphic trinucleotide repeats. Copyright © 1999 John Wiley & Sons, Ltd.