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Imaging time‐of‐flight secondary ion mass spectrometry of solid‐phase peptide syntheses
Author(s) -
Aubagnac J.L.,
Enjalbal C.,
Drouot C.,
Combarieu R.,
Martinez J.
Publication year - 1999
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199907)34:7<749::aid-jms828>3.0.co;2-p
Subject(s) - chemistry , mass spectrometry , protein mass spectrometry , peptide , ion , time of flight , phase (matter) , chromatography , secondary ion mass spectrometry , tandem mass spectrometry , organic chemistry , biochemistry
Imaging time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) of solid‐phase peptide syntheses carried out by the Merrifield and Sheppard strategies is described. Mixtures of resin beads mixed at random from batch syntheses or obtained in combinatorial chemistry by the mix and split technique, where each bead is functionalized by a unique peptide, were analyzed directly without any chemical cleavage of the growing chains to assess the nature of the growing structure on any bead of the mixture without its isolation. Copyright © 1999 John Wiley & Sons, Ltd.