Premium
Combining solid‐phase preconcentration, capillary electrophoresis and off‐line matrix‐assisted laser desorption/ionization mass spectrometry: intracerebral metabolic processing of peptide E in vivo
Author(s) -
Zhang Haying,
Stoeckli Markus,
Andren Per E.,
Caprioli Richard M.
Publication year - 1999
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199904)34:4<377::aid-jms778>3.0.co;2-d
Subject(s) - chemistry , chromatography , mass spectrometry , capillary electrophoresis , in vivo , matrix assisted laser desorption/ionization , peptide , capillary electrophoresis–mass spectrometry , desorption , protein mass spectrometry , microdialysis , tandem mass spectrometry , biochemistry , electrospray ionization , microbiology and biotechnology , extracellular , organic chemistry , adsorption , biology
The in vivo metabolism of peptide E was studied in theanesthetized rat using a combination of microdialysis sampling,solid‐phase preconcentration capillary electrophoresis andimaging matrix‐assisted laser desorption/ionization massspectrometry (MALDI/MS). The metabolic profile ofpeptides identified by MALDI/MS showed that the primary enzymaticactivity for degradation of peptide E was due to carboxypeptidasesand, to a lesser extent, aminopeptidases and some trypsin‐likeendopeptidases. Over 75 metabolic fragments were detected from theaction of these enzymes in vivo . Copyright © 1999 JohnWiley & Sons Ltd.