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Matrix optimization for matrix‐assisted laser desorption/ionization mass spectrometry of oligosaccharides from human milk
Author(s) -
Pfenninger Anja,
Karas Michael,
Finke Berndt,
Stahl Bernd,
Sawatzki Günther
Publication year - 1999
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199902)34:2<98::aid-jms767>3.0.co;2-n
Subject(s) - chemistry , mass spectrometry , chromatography , matrix assisted laser desorption/ionization , desorption , surface enhanced laser desorption/ionization , fragmentation (computing) , matrix (chemical analysis) , oligosaccharide , ionization , protein mass spectrometry , analytical chemistry (journal) , sample preparation , sample preparation in mass spectrometry , electrospray ionization , ion , organic chemistry , adsorption , computer science , operating system
Neutral and acidic oligosaccharides from human milk were analyzed by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI/MS). These experiments require suitable matrices; their selection and particularly their preparation protocols must be optimized. Important criteria are sensitivity, reproducibility, tolerance against impurities and resolution over a wide mass range. For analytical investigations of these oligosaccharides, containing labile fucosylated and sialylated components, another property of a matrix becomes a significant factor, namely the influence on ion stability and the extent of (metastable) fragmentation. The experience gained with the MALDI/MS of neutral and acidic oligosaccharides is summarized taking into account different intentions of measurement and typical problems, such as impurities after enzymatic treatment. For a rapid screening of an oligosaccharide sample, superior results were obtained with a new preparation technique using 5‐chloro‐2‐mercaptobenzothiazole (CMBT) as the first layer for 2,5‐dihydroxybenzoic acid. For structural analysis by post‐source decay, CMBT as the first layer for 3‐aminoquinoline is a favoured preparation protocol, because extensive fragmentation is achieved. For acidic oligosaccharides, a special preparation protocol makes it possible to determine the number of sialic acids by inducing highly effective cationization. matrix‐assisted laser desorption/ionization mass spectrometry; matrices; oligosaccharides; post‐source decay. Copyright © 1999 John Wiley & Sons, Ltd.