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Characterization of olanzapine (LY170053) in human liver slices by liquid chromatography/tandem mass spectrometry
Author(s) -
Murphy A. T.,
Lake B. G.,
Bernstein J. R.,
Franklin R. B.,
Gillespie T. A.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199812)33:12<1237::aid-jms742>3.0.co;2-f
Subject(s) - chemistry , glucuronide , olanzapine , chromatography , metabolite , mass spectrometry , glucuronidation , liquid chromatography–mass spectrometry , conjugate , human liver , biochemistry , in vitro , microsome , mathematical analysis , schizophrenia (object oriented programming) , mathematics , computer science , programming language
Olanzapine metabolism was investigated using incubation ofolanzapine with human liver slices. The intent of the investigationwas to identify olanzapine metabolites and determine if the humanliver slice incubations could potentially produce quantities of theolanzapine glucuronides for future studies. Along with known Phase 1olanzapine metabolites, N‐desmethyl‐,2‐hydroxymethyl‐, and4′‐N‐oxide‐, a new hydroxylated species wasdetected. Detection of Phase 2 metabolites included knownN‐10‐glucuronides, a quaternary glucuronide and a novelglucuronide conjugate. This investigation showed the feasibility ofusing human liver slices to produce sufficient quantities ofolanzapine glucuronides for further studies. © 1998 John Wiley& Sons, Ltd.