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Optimization of a hydrophobic solid‐phase extraction interface for matrix‐assisted laser desorption/ionization
Author(s) -
Brockman Adam H.,
Shah Ni.,
Orlando Ron
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(1998110)33:11<1141::aid-jms732>3.0.co;2-u
Subject(s) - chemistry , analyte , desorption , chromatography , matrix assisted laser desorption/ionization , solid phase extraction , analytical chemistry (journal) , extraction (chemistry) , ionization , matrix (chemical analysis) , dilution , adsorption , organic chemistry , ion , physics , thermodynamics
Matrix‐assisted laser desorption/ionization(MALDI) probe surfaces derivatized with octadecanethiol(C 18 ) can be used as hydrophobicsolid‐phase extraction devices to isolate and desaltbiopolymers directly on the probe surface. Using quantitative MALDI,it was possible to determine the approximate amount of peptide thatbound to C 18 surfaces and thus to calculate a surfacedensity. It was determined that the amount of peptide bound at theprobe surface was independent of the analyte concentration in theimmersion solution (from high‐ to sub‐ngml ‐1 concentrations), but rather was dependenton the immersion time of the surface as it was exposed to the analyte.The capacity of C 18 ‐derivatized probes to bindbiopolymers in fixed amounts frees the analyst from the necessity foradjusting analyte concentration through multiple step procedures suchas serial dilution or vacuum drying. This time savings result in anoverall increase in the efficiency of the MALDI technique. © 1998John Wiley & Sons, Ltd.