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Application of time‐of‐flight secondary ion mass spectrometry to in situ monitoring of solid‐phase peptide synthesis on the Multipin TM system
Author(s) -
Aubagnac J.L.,
Enjalbal C.,
Subra G.,
Bray A. M.,
Combarieu R.,
Martinez J.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(1998110)33:11<1094::aid-jms726>3.0.co;2-8
Subject(s) - chemistry , mass spectrometry , combinatorial chemistry , in situ , solid phase synthesis , ion , characterization (materials science) , peptide synthesis , peptide , chromatography , analytical chemistry (journal) , organic chemistry , nanotechnology , materials science , biochemistry
In the rapidly growing field of combinatorial chemistry, theMultipin approach has been used for rapid and efficient multipleparallel syntheses of organic compounds. This strategy is particularlywell adapted for the optimization of reaction conditions prior tochemical library syntheses, for the preparation of a wide range ofcompounds in relation to a lead structure or for the generation ofparallel libraries involving complex multistep chemistries. In allcases, direct in situ monitoring of support‐boundproducts would be highly valuable. In this work,time‐of‐flight secondary ion mass spectrometry wasapplied to the analysis of support‐bound intermediates. Theanalytical method was applied to a range of crowns loaded with variousdipeptides. The solid support was subjected to primary ion bombardmentand characteristic ions indicative of both the peptide chain and thepolymeric support were unambiguously identified. Analysis could beperformed at any stage of the synthesis, and as the method iseffectively non‐destructive, the analyzed crowns could befurther used to prepare target compounds. © 1998 John Wiley &Sons, Ltd.