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Basic matrices in the analysis of non‐covalent complexes by matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Jespersen S.,
Niessen W. M. A.,
Tjaden U. R.,
van der Greef J.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199811)33:11<1088::aid-jms725>3.0.co;2-8
Subject(s) - chemistry , covalent bond , mass spectrometry , desorption , matrix assisted laser desorption/ionization , matrix (chemical analysis) , ionization , analytical chemistry (journal) , chromatography , organic chemistry , ion , adsorption
A number of potential matrix candidates were investigated withregard to the importance of the pH in the matrixassisted laserdesorption/ionization mass spectrometry (MALDI/MS)analysis of non‐covalently bound protein complexes. Thematrices examined were 2,5‐dihydroxybenzoic acid(DHB), 4‐hydroxy‐α‐cyanocinnamicacid (HCCA), 2‐aminonicotinic acid (ANA),4‐nitroaniline (NA),2‐amino‐4‐methyl‐5‐nitropyridine(AMNP) and 3‐hydroxypicolinic acid (HPA).In solution these matrix compounds permitted the preparation of MALDIsamples at pH in the range 2–7. Among the matrices tested,complex formation, by specific non‐covalent interactions, couldonly be observed when HPA (pH 3.8) was used as the matrixfor the MALDI analysis. Under these conditions, specificnon‐covalent complex formation of recombinant streptavidin andglutathione‐ S ‐transferases were observed but notfor human hemoglobin. The MALDI spectra obtained with the neutralcompounds ANA (pH 4.4), NA (pH 6.4) and AMNP(pH 7.1) as matrices contain only peaks of the subunit withno signal of the non‐covalent bound complexes present.Considering the results obtained in this study with basic andacidified matrix materials, there does not appear to be a strongcorrelation between the pH of the matrix solution and the utility of amatrix for the analysis of non‐covalently bound complexes.© 1998 John Wiley & Sons, Ltd.