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Characterization of enzymatic pectin digests by matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Körner Roman,
Limberg Gerrit,
Mikkelsen Joørn Dalgaard,
Roepstorff Peter
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199809)33:9<836::aid-jms694>3.0.co;2-2
Subject(s) - chemistry , pectin , chromatography , mass spectrometry , desorption , degree of polymerization , sample preparation in mass spectrometry , matrix assisted laser desorption/ionization , protein mass spectrometry , tandem mass spectrometry , polymerization , electrospray ionization , organic chemistry , biochemistry , polymer , adsorption
The use of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for the characterization of partially methyl‐esterified enzymatic pectin digests is described. The sensitivities of several matrices, positive and negative ion modes and desalting techniques for these acidic oligosaccharides were compared. The most favorable results were obtained with a thin‐layer preparation of a mixture of 2,4,6‐trihydroxyacetophenone and nitrocellulose in the negative ion mode. Results are presented demonstrating the sensitive characterization of separated and unseparated high‐ester pectin digests obtained after complete digestion using Aspergillus niger pectin lyase and the analysis of digests after chemical modification. In the case of unseparated digests, the analysis of methylation patterns is demonstrated. Oligomers with a degree of polymerization up to 40 were detected after enrichment of large oligomers by propan‐2‐ol precipitation. © 1998 John Wiley & Sons, Ltd.