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Characterization of β‐lactams by photodissociation and collision‐activated dissociation in a quadrupole ion trap
Author(s) -
Goolsby Brian J.,
Brodbelt Jennifer S.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199808)33:8<705::aid-jms679>3.0.co;2-2
Subject(s) - infrared multiphoton dissociation , chemistry , quadrupole ion trap , photodissociation , dissociation (chemistry) , fragmentation (computing) , ion trap , tandem mass spectrometry , electrospray ionization , ion , mass spectrometry , collision induced dissociation , electrospray , photochemistry , analytical chemistry (journal) , chromatography , organic chemistry , computer science , operating system
Electrospray ionization was used to introduce β‐lactams, including cephalosporin and penicillin analogs, into a quadrupole ion trap for analysis by collision‐activated dissociation (CAD) and infrared multiphoton dissociation (IRMPD). The two dissociation methods provided similar spectra; however, photodissociation requires substantially less tuning than is typically required to optimize CAD. Moreover, IRMPD was effective even at the elevated pressures introduced by the electrospray source. Both CAD and IRMPD promote cleavage across the β‐lactam ring, resulting in highly diagnostic fragmentation patterns. Time‐resolved and SWIFT methods were used to determine fragmentation genealogies of the ions created by IRMPD. © 1998 John Wiley & Sons, Ltd.