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Calibration of isotope ratio mass spectrometry working standard for 2 H/ 1 H ratio analysis
Author(s) -
Lee W.N. Paul,
Lim Shu,
Bassilian Sara,
Bergner E. Anne
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199807)33:7<627::aid-jms677>3.0.co;2-7
Subject(s) - chemistry , isotope dilution , deuterium , mass spectrometry , analytical chemistry (journal) , calibration curve , calibration , isotope , hydrogen , isotope ratio mass spectrometry , oxide , chromatography , dilution , detection limit , organic chemistry , nuclear physics , thermodynamics , physics , statistics , mathematics
A method is described for the calibration of an isotope ratio mass spectrometry working standard for 2 H/ 1 H analysis using highly purified (>99.99% enriched) deuterium oxide ( 2 H 2 O). Serial dilution of such a compound can be used to construct a standard curve for calibration purposes using the relationship APE /100=δ D (SA) WR ‐δ D (BKG) WR 1000× r WR . where APE is the atom percent excess. It was found that the determined r WR had a precision of ±1%. Most of the variation came from sample handling, which includes the serial dilution of the weighed deuterium oxide, the reduction of water to hydrogen gas and the introduction of the hydrogen gas into the mass spectrometer. The use of this calibration procedure allows the direct determination of the isotope ratio r SA , and APE from a standard curve over a wide range of enrichment. Unlike the reference standards V‐SMOW and SLAP, the highly purified deuterium oxide is non‐exhaustible and readily available. The highly purified deuterium oxide should be used in place of secondary standards to calibrate working references for 2 H/ 1 H analysis in biological studies. © 1998 John Wiley & Sons, Ltd.