Premium
Recent progress in high‐performance anion‐exchange chromatography/ionspray mass spectrometry for molecular mass determination and characterization of carbohydrates using static and scanning array detection
Author(s) -
van der Hoeven R. A. M.,
Tjaden U. R.,
van der Greef J.,
van Casteren W. H. M.,
Schols H. A.,
Voragen A. G. J.,
Bruggink C.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199804)33:4<377::aid-jms647>3.0.co;2-q
Subject(s) - chemistry , mass spectrometry , chromatography , molecular mass , oligomer , degree of polymerization , detection limit , analytical chemistry (journal) , polymerization , biochemistry , organic chemistry , enzyme , polymer
Position‐ and time‐resolved ion counting (PATRIC) array detection with a magnetic sector mass spectrometer equipped with an ionspray interface in the on‐line coupling of high‐performance anion‐exchange chromatography (HPAEC) with mass spectrometry (MS) is described. PATRIC array detection is used in both the static and scanning mode for molecular mass determination and structure characterization caused by nozzle/skimmer‐induced fragmentation in the ionspray interface. The virtual mass range is extended to over 7000 Da by the presence of triply and quadruply sodiated molecules in an enzyme digest of an exocellular polysaccharide (EPS) of Lactococcus lactis and quadruply sodiated molecules of β‐1,4‐xylose oligomers. After optimization of the HPAEC/MS system, molecular mass information could be obtained down to ∽700 pg per injection for each α‐1,4‐glucose oligomer up to a degree of polymerization of 7 using PATRIC array detection in the static mode. Structure information was obtained for α‐1,4 glucose oligomers, galacturonic acid oligomers and an EPS digest using PATRIC array detection in the scanning mode. © 1998 John Wiley & Sons, Ltd.