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Hydrogen isotope determination for small‐size water samples using an equilibration technique
Author(s) -
Thielecke Frank,
Brand Willi,
Noack Rudolf
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199804)33:4<342::aid-jms640>3.0.co;2-s
Subject(s) - chemistry , deuterium , isotope , volume (thermodynamics) , analytical chemistry (journal) , isotope ratio mass spectrometry , range (aeronautics) , limiting , hydrogen , sample (material) , doubly labeled water , fractionation , oxygen isotope ratio cycle , isotopes of oxygen , chromatography , mass spectrometry , thermodynamics , nuclear physics , nuclear chemistry , organic chemistry , mechanical engineering , biochemistry , physics , materials science , basal metabolic rate , engineering , composite material
The hydrogen/deuterium/oxygen (H/D/O) equilibration technique for measuring isotope ratios has proved to be of great interest for a number of applications in addition to the doubly labelled water (DLW) method, which measures energy expenditure in free living subjects. However, the limiting factor of the sample volume represents a common problem in many of these methodologies. By utilizing a fully automated sample preparation device, water samples with different volumes were analysed with respect to the 2 H/ 1 H isotope ratios. The sample volume ranged from 4–0.25 ml. It was demonstrated that high precision was obtainable over the entire range. Although fractionation did seem to occur, it remained insignificant. Therefore, subsequent mass correction is possible and isotope ratios can be reliably obtained from water samples as small as 0.25 ml. This implies that the fully automated H/D/O equilibration technique is a valuable and useful tool for an extended range of research areas, in particular where the sample volume is limited. © 1998 John Wiley & Sons, Ltd.