Premium
Electrospray ionization mass spectrometry of metalloporphyrins
Author(s) -
Vandell Victor E.,
Limbach Patrick A.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199803)33:3<212::aid-jms619>3.0.co;2-e
Subject(s) - chemistry , electrospray ionization , mass spectrometry , extractive electrospray ionization , electrospray , protein mass spectrometry , sample preparation in mass spectrometry , electrospray mass spectrometry , chromatography , ion mobility spectrometry–mass spectrometry , direct electron ionization liquid chromatography–mass spectrometry interface , ambient ionization , ionization , chemical ionization , organic chemistry , ion
The magnesium, nickel, copper, zinc and vanadium metalloporphyrins from octaethylporphyrin, etioporphyrin I and tetraphenylporphyrin were characterized using electrospray ionization mass spectrometry (ESI‐MS). The ion abundance of each of the porphyrins present in binary mixtures was monitored as a function of the porphyrin concentration and is dependent on the metalloporphyrin oxidation potential. It was found that, for binary mixtures of metalloporphyrins whose oxidation potentials differ by less than 0.1 V, the resulting ion abundance of each species is directly proportional to the concentration of each analyte in the mixture. For binary mixtures whose oxidation potentials differ by more than 0.1 V, relative abundances of the radical cations of each metalloporphyrin are determined by the oxidation potential and concentration of each metalloporphyrin with the analyte of lowest oxidation potential being ionized preferentially. The ability to ionize selectively one porphyrin over another in a binary mixture offers the potential to use ESI‐MS for the qualitative analysis of porphyrins present in complex mixtures. © 1998 John Wiley & Sons, Ltd.