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In vivo microdialysis and liquid chromatography/thermospray mass spectrometry of the novel anticonvulsant 2,3:4,5‐bis‐ o ‐(1‐methylethylidene)‐β‐ D ‐fructopyranose sulfamate (topiramate) in rat brain fluid
Author(s) -
Masucci John A.,
Ortegon Marta E.,
Jones William J.,
Shank Richard P.,
Caldwell Gary W.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199801)33:1<85::aid-jms612>3.0.co;2-r
Subject(s) - chemistry , microdialysis , thermospray , chromatography , topiramate , anticonvulsant , mass spectrometry , high performance liquid chromatography , extracellular fluid , detection limit , selected reaction monitoring , tandem mass spectrometry , extracellular , biochemistry , epilepsy , neuroscience , biology
The concentration of a novel anticonvulsant, 2,3:4,5‐bis‐ O ‐(1‐methylethylidene)‐β‐ D ‐fructopyranose sulfamate (topiramate), was determined in the extracellular fluid of rat brain by in vivo microdialysis combined off‐line with liquid chromatography/thermospray mass spectrometry. A microdialysis probe was stereotaxically implanted in the nucleus accumbens region of the rat brain. The maximum concentration of topiramate in the brain dialysate for a dose of 50 mg kg ‐1 i.v. was ∽10 μ M and occurred 45 min post‐injection. The detection limit of topiramate in the extracellular fluid of rat brain was in the 0.1 μ M range using selected ion monitoring techniques. The base peak, which was the ammonium adduct ion [M+NH 4 ] + , was used for detection. An internal standard of d 12 ‐labeled topiramate was utilized for quantitation by isotope dilution analysis. © 1998 John Wiley & Sons, Ltd.