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Probing the energetics of charge‐remote fragmentation in carbocyanine dyes using collision‐ and surface‐induced dissociation mass spectrometry
Author(s) -
Melnyk Michael C.,
Carlson Richard E.,
Busch Kenneth L.,
Schey Kevin L.,
Bartlett Michael G.
Publication year - 1998
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199801)33:1<75::aid-jms611>3.0.co;2-v
Subject(s) - chemistry , fragmentation (computing) , dissociation (chemistry) , mass spectrum , mass spectrometry , collision induced dissociation , ion , electrospray ionization , molecule , analytical chemistry (journal) , alkyl , ionization , photochemistry , tandem mass spectrometry , chromatography , organic chemistry , computer science , operating system
Carbocyanine dyes used as molecular probes are symmetric ring compounds with long alkyl chains on each ring proximate to a resonance‐stabilized charge site. They provide high‐quality positive‐ion liquid second (LSI) and electrospray ionization mass spectra in which the intact cation is the dominant ion in the mass spectrum. The intact cation of a carbocyanine dye undergoes charge‐remote fragmentation (CRF), evident in the LSI mass spectra. CRF is also observed in collision‐induced dissociation and surface‐induced dissociation of the mass‐selected intact cation. Differential CRF processes from the two chains in the molecule are observed with increasing internal energy deposition. © 1998 John Wiley & Sons, Ltd.