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Rapid 18 O analysis of CO 2 samples by continuous‐flow isotope ratio mass spectrometry
Author(s) -
Schoeller D. A.,
Luke A. H.
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199712)32:12<1332::aid-jms598>3.0.co;2-e
Subject(s) - chemistry , isotope ratio mass spectrometry , inlet , mass spectrometry , analytical chemistry (journal) , δ18o , stable isotope ratio , continuous flow , isotope analysis , natural abundance , isotope , chromatography , sample (material) , sample preparation , ecology , mechanics , mechanical engineering , physics , quantum mechanics , engineering , biology
Continuous‐flow isotope ratio mass spectrometry (CF‐IRMS) has been shown to minimize sample preparation and reduce the sample size requirements and time for the determination of carbon and nitrogen. An inexpensive CF inlet was developed for the determination of the 18 O isotopic abundance in CO 2 . Initial efforts were hampered by significant exchangeable oxygen within the inlet system, which was minimized by design changes and post‐analysis mathematical correction. The system was tested for accuracy in the measurement of 18 O abundance in standards and was accurate to 0.04‰ with a precision of 0.03‰. Additional tests were performed using urinary water for the measurement of energy expenditure by the doubly labeled water method and the results agreed to within 1% with those from traditional off‐line, dual‐inlet IRMS. The CF‐IRMS reduced the analysis time to 2 min per sample compared with 15 min for traditional duel‐inlet IRMS. © 1997 John Wiley & Sons, Ltd.