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The influence of strongly acidic groups on the protonation of peptides in electrospray MS
Author(s) -
Svoboda Michal,
Meister Walter,
Kitas Eric A.,
Vetter Walter
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199711)32:10<1117::aid-jms568>3.0.co;2-d
Subject(s) - chemistry , protonation , electrospray , electrospray mass spectrometry , electrospray ionization , chromatography , stereochemistry , mass spectrometry , organic chemistry , ion
In order to obtain experimental data on the question of compensation of positive charges by anionic groups in multiply charged ions of polyfunctional molecules in electrospray MS, several pairs of peptides with the same basic structure but differentiated by one or two strongly acidic groups (phosphate or sulfonate) were investigated. It was found that depending on the density of basic centers and the strength of acidic groups present, the observed changes upon introduction of acidic groups ranged from complete elimination of the most highly charged state to absence of any difference. © 1997 John Wiley & Sons, Ltd.