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Determination of low 13 C‐glutamine enrichments using gas chromatography–combustion–isotope ratio mass spectrometry
Author(s) -
Menand Corinne,
Pouteau Etienne,
Marchini Sergio,
Maugère Pascale,
Krempf Michel,
Darmaun Dominique
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199711)32:10<1094::aid-jms563>3.0.co;2-w
Subject(s) - chemistry , mass spectrometry , chromatography , isotope ratio mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , combustion , glutamine , organic chemistry , amino acid , biochemistry
Glutamine is an essential fuel for tissues with high rates of cell replication, such as enterocytes and lymphocytes. Infusion of 13 C‐labeled glutamine tracers allows for measurement of the rates of production, utilization and oxidation of glutamine’s carbon skeleton in vivo . The use of this tracer, however, has been limited by its high cost and/or the difficulty in measuring low enrichments in biological fluids using conventional gas chromatography–mass spectrometry (GC/MS) techniques. We have developed a method using gas chromatography–combustion–isotope ratio mass spectrometry (GC–C–IRMS) that allows for the determination of low 13 C enrichments (down to 0.06 mol.% excess) with a precision of 2% or better, and a within‐day and between‐day variability better than 5%, in plasma free glutamine. The method was applied to measuring the incorporation of 13 C in plasma glutamine over the course of infusion of 13 C‐labeled acetate in a human subject. © 1997 John Wiley & Sons, Ltd.