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Quantification of Glycated Hemoglobin by Electrospray Ionization Mass Spectrometry †
Author(s) -
Nakanishi Toyofumi,
Miyazaki Ayako,
Kishikawa Masahiko,
Yasuda Mamoru,
Tokuchi Yoshiyuki,
Kanada Yasutaka,
Shimizu Akira
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199707)32:7<773::aid-jms533>3.0.co;2-y
Subject(s) - chemistry , chromatography , electrospray ionization , formic acid , glycated hemoglobin , mass spectrometry , analytical chemistry (journal) , diabetes mellitus , medicine , type 2 diabetes , endocrinology
Glycated hemoglobin, considered to be the best index for the treatment of diabetes mellitus, was measured by electrospray ionization mass spectrometry (ESI/MS) according to the method proposed by Morris et al . at the 44th ASMS Conference on Mass Spectrometry and Allied Topics, 1996. They compared the values obtained by MS and affinity chromatography. Here, the values obtained by ESI/MS were compared with those obtained by high‐performance liquid chromatography and by latex agglutination immunoassay. Whole blood samples were diluted 500 fold with 0.2% formic acid–50% acetonitrile solution and 5 μl of the diluted solution was injected with the ESI/MS system (TSQ 7000) via a sample loop. The within‐run and between‐run relative standard deviations of the ratio of glycated and non‐glycated β‐chain were less than 5%. The correlation coefficients between ESI/MS and conventional methods were higher than 0.96. However, considerable discrepancies were observed among methods. ESI/MS will allow reproducible measurements of glycated hemoglobin and will be useful in the quality control of HbA1c measurement by other principles and also in routine clinical laboratory tests. © 1997 by John Wiley & Sons, Ltd.