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Profile and Flight Time Analysis of Bovine Insulin Clusters as a Probe of Matrix‐assisted Laser Desorption/Ionization Ion Formation Dynamics
Author(s) -
Kinsel Gary R.,
Edmondson Ricky D.,
Russell David H.
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199707)32:7<714::aid-jms523>3.0.co;2-1
Subject(s) - chemistry , ion , ionization , mass spectrometry , soft laser desorption , desorption , cluster (spacecraft) , ambient ionization , matrix assisted laser desorption electrospray ionization , yield (engineering) , time of flight mass spectrometry , matrix assisted laser desorption/ionization , atomic physics , analytical chemistry (journal) , mass spectrum , chemical physics , chemical ionization , electron ionization , chromatography , thermodynamics , physics , organic chemistry , adsorption , computer science , programming language
Detailed ion signal profile and flight time analyses were performed on the time‐of‐flight signals obtained for bovine insulin cluster ions produced by matrix‐assisted laser desorption/ionization (MALDI). Profile analyses of the ion signals strongly suggest that the signals are made up of a composite of two separate components and analysis of the flight times of the two components suggests that the ions are formed with different dynamics. The formation dynamics of one component of the ions is best described as prompt ionization to yield ions with essentially mass‐independent total energies. The formation dynamics of the second component of the ions is best described as delayed gas‐phase ionization of entrained material moving at a constant velocity. This model is supported by several ancillary experiments and suggests new insights into the mechanism of MALDI ion formation. © 1997 by John Wiley & Sons, Ltd.