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Evaluation of an Automated Equilibration Technique for Deuterium/Hydrogen Isotope Ratio Measurements with Respect to Assessing Total Energy Expenditure by the Doubly Labelled Water Method
Author(s) -
Thielecke F.,
Noack R.
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199703)32:3<323::aid-jms491>3.0.co;2-x
Subject(s) - doubly labeled water , chemistry , deuterium , isotope dilution , energy expenditure , matrix (chemical analysis) , isotope , linearity , analytical chemistry (journal) , dilution , hydrogen , energy (signal processing) , chromatography , statistics , thermodynamics , mass spectrometry , atomic physics , biochemistry , nuclear physics , medicine , physics , basal metabolic rate , quantum mechanics , endocrinology , mathematics , organic chemistry
The doubly labelled water (DLW) method for measuring energy expenditure in free living subjects suffers from inaccuracy and imprecision mainly owing to analytical problems. An automated H/D/O equilibration device was tested for precision, internal validity and matrix effects with particular consideration of DLW studies. The precision was found to be 0.5 ‰ and 0.04 ‰ for 2 H and 18 O, respectively. Dilution series with water standards and urine showed a high linearity and no matrix effects. In addition, the automated preparation is less laborious and time consuming and reduces sources of analytical imprecisions in studies measuring energy expenditure by the DLW method. © 1997 by John Wiley & Sons, Ltd.