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Continuous Flow Liquid Secondary Ion Mass Spectrometric Characterization of Phospholipid Molecular Species
Author(s) -
Li Chun,
Yergey James A.
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199703)32:3<314::aid-jms494>3.0.co;2-m
Subject(s) - chemistry , phospholipid , chromatography , ion , molecular mass , detection limit , analytical chemistry (journal) , capillary action , mass spectrometry , membrane , organic chemistry , biochemistry , composite material , materials science , enzyme
The positive and negative ion continuous flow liquid secondary ion mass spectrometric (CF‐LSIMS) analysis of four major phospholipid classes is reported. Coupling capillary high‐performance liquid chromatography with CF‐LSIMS proved to be very useful for the structural identification of minor phospholipid species present in complex mixtures. Phospholipid molecular species within a particular class were separated or partially separated on the capillary column. Abundant molecular ions and characteristic fragment ions were produced. Information regarding the molecular mass, the polar head group and the fatty acyl substituents can be easily obtained, with a limit of detection below 1 ng per component. © 1997 by John Wiley & Sons, Ltd.