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β‐Carboline Alkaloids as Matrices for Matrix‐assisted Ultraviolet Laser Desorption Time‐of‐flight Mass Spectrometry of Proteins and Sulfated Oligosaccharides: a Comparative Study Using Phenylcarbonyl Compounds, Carbazoles and Classical Matrices
Author(s) -
ami Hiroshi,
Fukui Shinsaku,
ErraBalsells Rosa
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199703)32:3<287::aid-jms478>3.0.co;2-#
Subject(s) - chemistry , mass spectrometry , sulfation , chromatography , matrix assisted laser desorption/ionization , matrix (chemical analysis) , time of flight mass spectrometry , desorption , organic chemistry , biochemistry , ion , ionization , adsorption
The successful use of six β‐carbolines (i.e. nor ‐harmane (9H‐pyrido[3,4‐ b ]indole), harmane (1‐methyl‐9 H ‐pyrido[3,4‐ b ]indole), harmine (7‐methoxy‐1‐methyl‐9 H ‐pyrido[3,4‐ b ]indole), harmol (1‐methyl‐9 H ‐pyrido[3,4‐ b ]indol‐7‐ol), harmaline (3,4‐dihydro‐7‐methoxy‐1‐methyl‐9 H ‐pyrido[3,4‐ b ]indole) and harmalol (3,4‐dihydro‐1‐methyl‐9 H ‐pyrido[3,4‐ b ]indol‐7‐ol)) is reported as matrices in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI/TOF‐MS) (λ exc =337 nm) for proteins (proteins checked: gramicidin S, bovine insulin, aprotinin, horse heart cytochrome c , ribonuclease A, lysozyme, myoglobin, trypsin, protease and bovine serum albumin) and sulfated oligosaccharides (λ‐carrageenans of M r 549, 712, 1570 and 1733), using stainless‐steel probes and membranes (poly(vinylidene difluoride) (PVDF) polymers) to prepare the samples. The possible use of some phenylcarbonyl compounds (hydroxyphenyl ketones, amino‐ and hydroxybenzoic acids) and a few carbazole derivatives as matrices is also discussed briefly. In addition, the usefulness of the new matrices in MALDI/TOF‐MS (λ exc =337 nm) of proteins and sulfated oligosaccharides is compared with those of classical MALDI matrices such as α‐cyano‐4‐hydroxycinnamic acid, gentisic acid, sinapinic acid, 6‐aza‐2‐thiothymine and 3‐indole‐ trans ‐β‐acrylic acid. Laser desorption/TOF‐MS (λ exc =337 nm) of the new matrices is also described. © 1997 by John Wiley & Sons, Ltd.