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Fast Atom Bombardment Mass Spectrometry for the Characterization of Taxanes
Author(s) -
Madhusudanan K. P.,
Chattopadhyay S. K.,
Saha G. C.,
Kulshrestha M.,
Sharma R. P.,
Kumar Sushil
Publication year - 1997
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199702)32:2<216::aid-jms473>3.0.co;2-s
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , methylene , adduct , dissociation (chemistry) , molecule , collision induced dissociation , oxetane , ion , fragmentation (computing) , stereochemistry , medicinal chemistry , tandem mass spectrometry , organic chemistry , chromatography , computer science , operating system
Fast atom bombardment mass spectrometry (FABMS) of several taxanes and 11(15→1) abeo ‐taxanes having a 4(20)‐exocyclic methylene group or oxetane ring was studied in detail using collision‐induced dissociation (CID) of their [M+Li] + , [M+Na] + and [M+NH 4 ] + ions. The results indicate that FAB followed by MS/MS is a valuable tool for the identification of functional groups in taxanes and to some extent the type of taxane under investigation. The presence of hydroxy groups in the molecule is best confirmed by examining the CID of the [M+NH 4 ] + adduct ions. The 11(15→1) abeo ‐taxanes are characterized by elimination of the hydroxyisopropylidene group with an acetoxy or benzoyloxy group. © 1997 by John Wiley & Sons, Ltd.