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Characterization of Cytokinins by Electrospray Ionization and Collision‐induced Dissociation Mass Spectrometry Without Precursor Ion Selection
Author(s) -
Bartók T.,
Börcsök G.,
Komoróczy R.,
Sági F.
Publication year - 1996
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199607)31:7<756::aid-jms348>3.0.co;2-l
Subject(s) - chemistry , mass spectrometry , fragmentation (computing) , electrospray ionization , protonation , collision induced dissociation , dissociation (chemistry) , ion , molecule , tandem mass spectrometry , analytical chemistry (journal) , electrospray , ionization , chromatography , organic chemistry , computer science , operating system
Eight naturally occurring and four synthetic cytokinins were studied by means of electrospray ionization collision‐induced dissociation single‐quadrupole mass spectrometry in order to examine the potential of this technique in the mapping of their structures. At a low capillary exit voltage, protonated molecular ions dominated, but in the range 240–300 V informative and typical collision‐induced fragmentation was obtained. At these voltages, protonated adenine and purine ions ( m/z 136 and 119, respectively) were formed from all natural but not from the synthetic cytokinins, which produced other ions specific to the molecule. Nevertheless, the loss of 132 u ([M+H‐C 5 H 8 O 4 ] + ) or 162 u ([M+H‐C 6 H 10 O 5 ] + ) pointed to the presence of the ribosyl or glucosyl group in the two types of cytokinins. The occurrence of the molecular and ‘diagnostic’ ions, and the possible loss of 132 or 162 u, readily permit the characterization of the chemical structure and the identification of unknown cytokinin molecules.