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Assay of the Deuterium Enrichment of Water via Acetylene
Author(s) -
Previs Stephen F.,
Hazey Jeffrey W.,
Diraison Frédérique,
Beylot Michel,
David France,
Brunengraber Henri
Publication year - 1996
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199606)31:6<639::aid-jms336>3.0.co;2-s
Subject(s) - chemistry , acetylene , deuterium , electron ionization , mass spectrometry , chemical ionization , ionization , ion source , analytical chemistry (journal) , hydrogen , gas chromatography , calcium carbide , molecule , ion , radiochemistry , chromatography , organic chemistry , atomic physics , physics
A technique is presented for measuring the 2 H enrichment of water in biological samples when this enrichment is greater than 0.2%. The sample is reacted with calcium carbide to form acetylene gas, which is determined by gas chromatography electron impact ionization mass spectrometry. Ion–molecule reactions, resulting in proton abstraction, are minimized by lowering the electron ionization energy from the usual 70 eV to 45 eV. This technique is much more rapid and economical than the classical isotope ratio mass spectrometric assay of the enrichment of hydrogen gas derived from reduction of water.