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Urinary Testosterone (T) To Epitestosterone (E) Ratios by GC/MS. I. Initial Comparison of Uncorrected T/E in Six International Laboratories
Author(s) -
Catlin D. H.,
Cowan D. A.,
de la Torre R.,
Donike M.,
Fraisse D.,
Oftebro H.,
Hatton C. K.,
Starcevic B.,
Becchi M.,
de la Torre X.,
Norli H.,
Geyer H.,
Walker C. J.
Publication year - 1996
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199604)31:4<397::aid-jms316>3.0.co;2-a
Subject(s) - epitestosterone , chemistry , chromatography , urine , context (archaeology) , testosterone (patch) , analytical chemistry (journal) , androgen , medicine , biochemistry , paleontology , hormone , biology
Six laboratories in six countries collaborated to investigate the analytical method for estimating the testosterone to epitestosterone ratio (T/E) in urine by gas chromatography/mass spectrometry in the context of detecting the application of T as a doping agent in sport. The protocol specified many but not all details of reagents and instrument conditions. The design included the distribution and analysis of four urines with different T/E values, three replicates per value, and one standard. The ranges of mean T/E values for the four urines estimated by peak area (PA) were 0.32–0.42, 0.72–0.94, 0.91–1.14 and 3.19–5.48. The analyses of variance for these data and for the peak height (PH) data were significant for the laboratory factor ( p< 0.0001). In addition there was a significant interaction between the urine factor and the laboratory factor which indicates the complexity of the analysis. T/E calculated using PA was not significantly different from that using PH. For within‐laboratory precision all values for PH and PA were <8.3%, and for between‐laboratory precision all values were <11.7% except for one (20.1%). The data represent a baseline for future experiments designed to elucidate the sources of within‐and between‐laboratory variance, and to harmonize estimates of T/E.