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Characterization and differentiation of isomeric self‐complementary DNA oligomers by electrospray tandem mass spectrometry
Author(s) -
Gentil E.,
Banoub J.
Publication year - 1996
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199601)31:1<83::aid-jms274>3.0.co;2-4
Subject(s) - chemistry , tandem mass spectrometry , mass spectrometry , electrospray , oligonucleotide , dna , tandem , dissociation (chemistry) , electrospray ionization , stereochemistry , chromatography , organic chemistry , biochemistry , materials science , composite material
Two series of synthetic self‐complementary isomeric DNA hexamers, namely d(CAGCTG), d(CGATCG) and d(CGTACG) ( M r 1791) and d(CATATG), d(TGATCA) and d(TGTACA) ( M r 1790) were analysed by negative electrospray mass spectrometry. As expected, these DNA hexamers exhibited identical series of multi‐charged deprotonated molecular ions. Low‐energy collision‐induced dissociation tandem mass spectrometric analysis of the multi‐charged oligonucleotide anions [M — 3H] 3− and [M — 4H] 4− provided characteristic and distinct fingerprint patterns which permitted discrimination amongst the individual isomeric DNA hexamers and allowed complete direct sequence determination.