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Binding specificity of post‐activated neocarzinostatin chromophore drug‐bulged DNA complex studied using electrospray ionization mass spectrometry
Author(s) -
Gao Quanyin,
Cheng Xueheng,
Smith Richard D.,
Yang Catherine F.,
Goldberg Irving H.
Publication year - 1996
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/(sici)1096-9888(199601)31:1<31::aid-jms244>3.0.co;2-i
Subject(s) - chemistry , electrospray ionization , dna , mass spectrometry , chromophore , neocarzinostatin , stereochemistry , electrospray , fluorescence , crystallography , photochemistry , chromatography , biochemistry , physics , quantum mechanics
Electrospray ionization mass spectrometry (ESI‐MS) was employed to characterize the binding specificity of a bulged 22‐mer DNA hairpin with a post‐activated neocarzinostatin chromophore (NCS‐Chrom) having two similar forms, where 2a has an H in a location for which 2b has it replaced by a CH 2 OH group. Specific binding of 2a to the bulged 22‐mer DNA was observed whereas little binding was detected for 2a to non‐bulged DNA 19‐mer and 12‐mer duplexes. The stoichiometry of the 22‐mer DNA complex with 2a was determined to be predominantly 1:1. Substitution of hydrogen in 2a for the hydroxymethylene group in 2b dramatically reduced the binding strength to the 22‐mer DNA. Little complex formation was observed for 2b and 22‐mer DNA based upon the ESI‐MS data, consistent with earlier fluorescence studies. The results indicate that ESI‐MS can be a sensitive technique for probing conformational specificity in studies of biomolecular binding.