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Human nuclear respiratory factor 2α subunit cDNA: Isolation, subcloning, sequencing, and in situ hybridization of transcripts in normal and monocularly deprived macaque visual system
Author(s) -
Guo Aili,
Nie Feng,
WongRiley Margaret
Publication year - 2000
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(20000207)417:2<221::aid-cne7>3.0.co;2-4
Subject(s) - ocular dominance column , in situ hybridization , biology , microbiology and biotechnology , lateral geniculate nucleus , cdna library , complementary dna , protein subunit , gene expression , visual cortex , neuroscience , gene , genetics , ocular dominance
Nuclear respiratory factor 2 (NRF‐2) has been shown to contribute to the transcriptional regulation of a number of subunits of respiratory chain enzymes, including cytochrome c oxidase (CO). Our recent study demonstrated a parallel distribution of the α subunit proteins of NRF‐2 (NRF‐2α) with CO in the monkey striate cortex, and that it can be regulated by neuronal activity. To determine whether this regulation is at the transcriptional level, the present study examined the expression of NRF‐2α mRNA in normal and monocularly deprived adult monkeys. A partial NRF‐2α cDNA was isolated from a human brain cDNA library. Sequence analysis revealed that it shared 99% identity with the published sequence from human HeLa cells. Riboprobes of NRF‐2α was generated and labeled with digoxigenin‐11‐UTP for in situ hybridization. The expression pattern of NRF‐2α mRNA in the normal striate cortex paralleled that of CO activity. It was highly expressed in layers IVC and VI, which contained high levels of CO, and more densely expressed in puffs of layers II and III than in interpuffs. In monkeys monocularly treated with tetrodotoxin for 1 day to 2 weeks, both NRF‐2α expression and CO activity were reduced in deprived ocular dominance columns of the visual cortex and in deprived layers of the lateral geniculate nucleus. These data indicate that, in the normal and visually deprived adult monkeys, NRF‐2α is regulated by neuronal activity at the transcriptional level. J. Comp. Neurol. 417:221–232, 2000. ©2000 Wiley‐Liss, Inc.