Premium
Subcellular distribution of 5‐hydroxytryptamine 2A and N ‐methyl‐ D ‐aspartate receptors within single neurons in rat motor and limbic striatum
Author(s) -
Rodríguez J.J.,
Garcia D.R.,
Pickel V.M.
Publication year - 1999
Publication title -
journal of comparative neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.855
H-Index - 209
eISSN - 1096-9861
pISSN - 0021-9967
DOI - 10.1002/(sici)1096-9861(19991018)413:2<219::aid-cne4>3.0.co;2-f
Subject(s) - biology , medium spiny neuron , neuroscience , nucleus accumbens , glutamate receptor , nmda receptor , dendritic spine , striatum , colocalization , forebrain , receptor , central nervous system , dopamine , hippocampal formation , biochemistry
The dorsolateral caudate‐putamen nucleus (CPN) and the nucleus accumbens (NAc) shell, respectively, are involved in many motor and limbic functions that are affected by activation of the 5‐hydroxytryptamine 2A receptor (5HT 2A R) and the N ‐methyl‐ D ‐aspartate subtype of glutamate receptor (NMDAR). We examined the functional sites for 5HT 2A R activation and potential interactions involving the NMDAR subunit NR1 (NMDAR1) within these striatal regions. For this examination, sequence‐specific antipeptide antisera against these receptors were localized by electron microscopic dual‐labeling immunocytochemistry in the rat brain. In the dorsolateral CPN and the NAc shell, the 5HT 2A R‐labeled profiles were mainly dendrites, but somata and axons were also immunoreactive. The neuronal somata contained round unindented nuclei that are typical of spiny striatal neurons, although few dendritic spines were 5HT 2A R immunolabeled. In all neuronal profiles, the 5HT 2A R labeling was primarily associated with cytoplasmic organelles and more rarely was localized to synaptic or nonsynaptic plasma membranes. Colocalization of 5HT 2A R and NMDAR1 was seen primarily in somata and dendrites. Significantly greater numbers of 5HT 2A R‐ or 5HT 2A R‐ and NMDAR1‐containing dendrites were seen in the dorsolateral CPN than in the NAc shell. As compared with 5HT 2A R, NMDAR1 labeling was more often observed in dendritic spines, and these were also more numerous in the CPN. These results indicate that 5HT 2A and NMDA receptors are coexpressed but differentially targeted in single spiny striatal neurons and are likely to play a major role in control of motor functions involving the dorsolateral CPN. J. Comp. Neurol. 413:219–231, 1999. © 1999 Wiley‐Liss, Inc.